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该系统具有一个恒温样品池,该恒温样品池直接耦合到具有吸收光谱和荧光光谱检测选项的光学系统。电容性放电电源用于存储迅速释放到样品中的高压能量。

信号检测系统集成了快速响应放大器和信号调节电子设备。使用Kinetic Studio进行数据采集和处理,它允许在线性时基和对数时基上进行数据采集以及显示和分析。可以设置多个跳转,并在Kinetic Studio的管理下以自动方式进行跳转。这种统整解决技术提供了一个相对简单易用的系统。样品池与大多数化学和生物系统兼容,并且可以在很宽的范围内进行恒温。

我们在为各种化学和生物系统设计和开发此类T-Jump设备方面拥有丰富的经验。有关更多信息或相关应用,请随时与我们联系。

参考出版期刊

  1. Gianni, S., Morrone, A., Giri, R. and Brunori, M. (2012) A folding-after-binding mechanism describes the recognition between the transactivation domain of c-Myb and the KIX domain of the CREB-binding protein. Biochemical and Biophysical Research Communications, 428, Issue 2, 16 November 2012, pp. 205-209.
  2. Giri, R., Morrone, A., Travaglini-Allocatelli, C., Jemth, P., Brunori, M. and Gianni, S. (2012) Folding pathways of proteins with increasing degree of sequence identities but different structure and function. PNAS, Early Edition.
  3. Dodson, C.A., Ferguson, N., Rutherford, T.J., Johnson, C.M. and Fersht, A. (2010) Engineering a two-helix bundle protein for folding studies. Protein Engineering, Design & Selection, 23, no. 5, pp. 357-364.
  4. Neuweiler, H., Johnson, C.M. and Fersht, A. (2009) Direct Observation of ultrafast folding and denatured state dynamics in single protein molecules. PNAS, November 3, 106, no. 44, pp. 18569-18574.
  5. Hart, T., Hosszu, L.L.P., Trevitt, C.R., Jackson, G.S., Waltho, J.P., Collinge, J. and Clarke, A.R. (2009) Folding kinetics of the human prion protein probed by temperature jump. PNAS, April 7, 106, no. 14, pp. 5651-5656.