紫外线可见光谱法非常适合于停流观测,并已确立为一种可信赖的实验方法。而红外光谱法在键结和轨域混成结构方面提供了更多的结构信息。
尽管实施起来较复杂,TgK Scientific设计出一套专为控制应用程序KinetaDrive,与已建立的FT-IR仪器供货商一起合作,开发出了一种具有短光径的停流解决方案(Stopped Flow for FT-IR)。
混合池已经可以进行 ATR 分析
范例中,放置在Bruker Vertex 80光谱仪中的 ATR混合池可兼容于 Specac Golden Gate 系列,是单反射式的ATR。这可对大多数的试剂与溶剂提供最佳兼容性与降低背景干扰。
参考出版期刊
- Paul A. Cox, Marc Reid, Andrew G. Leach, Andrew D. Campbell, Edward J. King, and Guy C. Lloyd-Jones. (2017) Base-catalyzed Aryl-B(OH)2 Protodeboronation Revisited: from Concerted Proton-Transfer to Liberation of a Transient Arylanion. J. Am. Chem. Soc., 2017, 139 (37), pp 13156–13165.
- Wright, J.A., Webster,L., Jablonskyte, A., Woi, P.M., Ibrahim, S.K. and Pickett, C.J. (2011) Protonation of [FeFe]-hydrogenase sub-site analogues: revealing mechanism using FTIR stopped-flow techniques. Faraday Discuss.,148, pp. 359-371.
- Jablonskyte, A., Wright, J.A. and Pickett, C.J. (2010) Mechanistic aspects of the protonation of [FeFe]-hydrogenase subsite analogues. Dalton Trans.,39, pp. 3026-3034.
- Wright, J.A. and Pickett, C.J. (2009) Protonation of a subsite analogue of [FeFe]-hydrogenase: mechanism of a deceptively simple reaction revealed by time-resolved IR spectroscopy. Chem. Commun., pp. 5719-5721.
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