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紫外线可见光谱法非常适合于停流观测,并已确立为一种可信赖的实验方法。而红外光谱法在键结和轨域混成结构方面提供了更多的结构信息。

尽管实施起来较复杂,TgK Scientific设计出一套专为控制应用程序KinetaDrive,与已建立的FT-IR仪器供货商一起合作,开发出了一种具有短光径的停流解决方案(Stopped Flow for FT-IR)。

 

混合池已经可以进行 ATR 分析

范例中,放置在Bruker Vertex 80光谱仪中的 ATR混合池可兼容于 Specac Golden Gate 系列,是单反射式的ATR。这可对大多数的试剂与溶剂提供最佳兼容性与降低背景干扰。

参考出版期刊

  1. Paul A. Cox, Marc Reid, Andrew G. Leach, Andrew D. Campbell, Edward J. King, and Guy C. Lloyd-Jones. (2017) Base-catalyzed Aryl-B(OH)2 Protodeboronation Revisited: from Concerted Proton-Transfer to Liberation of a Transient Arylanion. J. Am. Chem. Soc., 2017, 139 (37), pp 13156–13165.
  2. Wright, J.A., Webster,L., Jablonskyte, A., Woi, P.M., Ibrahim, S.K. and Pickett, C.J. (2011) Protonation of [FeFe]-hydrogenase sub-site analogues: revealing mechanism using FTIR stopped-flow techniques. Faraday Discuss.,148, pp. 359-371.
  3. Jablonskyte, A., Wright, J.A. and Pickett, C.J. (2010) Mechanistic aspects of the protonation of [FeFe]-hydrogenase subsite analogues. Dalton Trans.,39, pp. 3026-3034.
  4. Wright, J.A. and Pickett, C.J. (2009) Protonation of a subsite analogue of [FeFe]-hydrogenase: mechanism of a deceptively simple reaction revealed by time-resolved IR spectroscopy. Chem. Commun., pp. 5719-5721.

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